Production of n-butanol from biodiesel derived crude glycerol using Clostridium pasteurianum immobilized on Amberlite

► Study on conversion of biodiesel derived crude glycerol to n-butanol. ► Anaerobic fermentation using immobilized Clostridium pasteurianum cells. ► Cells immobilized on a hydrophilic support Amberlite, an ion exchange resin. ► Concentration of crude glycerol found to have significant effect on product profile. ► 25 g L−1 glycerol concentration gives maximum yield of n-butanol after 120 h.