کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6661535 | 1426551 | 2018 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A photoelectrochemical immunoassay for tumor necrosis factor-α using a GO-PTCNH2 nanohybrid as a probe
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
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چکیده انگلیسی
A simple photoelectrochemical (PEC) immunoassay based on an amino-terminated perylene derivative (PTCNH2) was developed for highly sensitive and specific detection of tumor necrosis factor-alpha (TNF-α). Simple grinding and sonication afforded a Ï-Ï stacked graphene oxide (GO)/PTCNH2 assembly exhibiting fast electron transfer kinetics and excellent photoelectric performance, and this assembly was applied as a PEC probe. The GO-PTCNH2 assembly provided not only a large surface area, fast electron transfer kinetics and abundant active sites for further biomolecule conjugation but also high PEC activity. A “signal-off” PEC immunoassay for TNF-α was developed by assembling GO-PTCNH2 on the electrode, followed by conjugation of anti-TNF-α with GO-PTCNH2 and subsequent capture of the target protein TNF-α. The linear detection range was from 10â¯pg/mL-100â¯ng/mL with a limit of detection (LOD) of 3.33â¯pg/mL. This method was also successfully applied for detecting TNF-α in human serum with recoveries ranging from 92.5% to 96.6% and a relative standard deviation of less than 9.99%. For the first time, this work reports a simple PEC immunoassay achieved by using PTC-NH2 as a photoelectrochemically active probe for the detection of a tumor biomarker. This assay could be applicable to the detection of other biomarkers for clinical diagnosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Electroanalytical Chemistry - Volume 824, 1 September 2018, Pages 195-200
Journal: Journal of Electroanalytical Chemistry - Volume 824, 1 September 2018, Pages 195-200
نویسندگان
Huizhen Hu, Deng Pan, Huaijia Xue, Mingming Zhang, Yuanjian Zhang, Yanfei Shen,