کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
681154 1460006 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Production of (3S)-acetoin from diacetyl by using stereoselective NADPH-dependent carbonyl reductase and glucose dehydrogenase
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Production of (3S)-acetoin from diacetyl by using stereoselective NADPH-dependent carbonyl reductase and glucose dehydrogenase
چکیده انگلیسی

Highlight
• A carbonyl reductase catalyzed NADPH-dependent reduction of DA to produce (3S)-AC.
• A glucose dehydrogenase was coupled with the carbonyl reductase to produce (3S)-AC from DA.
• (3S)-AC of 12.2 g l−1 was produced from DA of 14.3 g l−1 under optimal conditions.
• The two-enzymes coupling system provided a potential pathway for (3S)-AC production.

Production of (3S)-acetoin ((3S)-AC), an important platform chemical, is desirable but difficult to perform. An NADPH-dependent carbonyl reductase (Gox0644) from Gluconobacter oxydans DSM 2003 was confirmed to have a good ability to reduce diacetyl (DA) to produce (3S)-AC. In this work, the NADPH-dependent carbonyl reductase was expressed and purified. Glucose dehydrogenase from Bacillus subtilis 168 was coupled with the NADPH-dependent carbonyl reductase to produce (3S)-AC from DA. Under the optimal conditions, 12.2 g l−1 (3S)-AC was produced from 14.3 g l−1 DA in 75 min. Because DA can be biotechnological produced, the two-enzymes coupling system might be a promising alternative for the (3S)-AC production.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 137, June 2013, Pages 111–115
نویسندگان
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