کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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681342 | 1460018 | 2012 | 6 صفحه PDF | دانلود رایگان |
l-Asparaginase has potential as an anti-cancer drug and for prevention of acrylamide formation in fried and baked foods. Production of the enzyme by Bacillus licheniformis (RAM-8) was optimized by process engineering using a statistical modeling approach and a maximum yield of 32.26 IU/ml was achieved. The l-asparaginase exhibited glutaminase activity of only 0.8 IU/ml and would therefore be less prone to cause the side effects associated with asparaginase therapy compared to enzyme preparations with higher glutaminase activities. When production was carried out in a 30-L bioreactor, enzyme production reached 29.94 IU/ml in 15 h. The enzyme inhibited poly-acrylamide formation in 10% acrylamide solution and reduced acrylamide formation in fried potatoes by 80%.
► Production of l-asparaginase from Bacillus licheniformis and its statistical optimization.
► High yields of enzyme obtained i.e. 32 IU/ml in 18 h after statistical optimization.
► Optimized process yielded 30 IU/ml of enzyme in 15 h is obtained in 30 L fermenter.
► l-Asparaginse produced by B. licheniformis is free of glutaminase activity.
► l-Asparaginase produced was efficiently able to degrade polyacrylamide.
Journal: Bioresource Technology - Volume 125, December 2012, Pages 11–16