کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
681517 1460026 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Saccharification of woody biomass using glycoside hydrolases from Stereum hirsutum
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Saccharification of woody biomass using glycoside hydrolases from Stereum hirsutum
چکیده انگلیسی

Enzymatic saccharification of woody biomasses was performed using glycoside hydrolases from Stereum hirsutum, a newly isolated fungal strain found to secrete efficient glycoside hydrolases. The strain showed the highest β-glucosidase, cellobiohydrolase, endoglucanase, endoxylanase, laccase, and filter paper activity of 10.3, 1.7, 10.3, 29.9, 0.12, and 0.58 U/ml, respectively. Among the various biomasses tested for saccharification, pine biomass produced maximum reducing sugar. Response surface methodology was used to optimize the hydrolysis of pine biomass to achieve the highest level of sugars. The parameters including enzyme, substrate concentration, temperature and pH were found to be critical for the conversion of pine biomass into sugars. Maximum saccharification of 49.7% (435 mg/g-substrate) was obtained after 96 h of hydrolysis. A close agreement between the experimental results and the model predictions was achieved. S. hirsutum could be a good choice for the production of reducing sugars from cellulosic biomasses.


► It reports saccharification of woody biomasses using glycoside hydrolases from Stereum hirsutum, a newly isolated fungal strain.
► Among the biomasses tested, pine biomass produces maximum reducing sugar.
► Response surface methodology is used to optimize the hydrolysis of pine biomass to achieve the highest level of sugars.
► S. hirsutum can be a good choice for the production of reducing sugars from cellulosic biomasses.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 117, August 2012, Pages 310–316
نویسندگان
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