کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
681545 1460028 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Immobilization of laccase on magnetic bimodal mesoporous carbon and the application in the removal of phenolic compounds
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Immobilization of laccase on magnetic bimodal mesoporous carbon and the application in the removal of phenolic compounds
چکیده انگلیسی

A novel magnetically separable laccase immobilized system was constructed by adsorbing laccase into bimodal carbon-based mesoporous magnetic composites (CMMC). A large adsorption capacity (491.7 mg g−1), excellent activity recovery (91.0%) and broader pH and temperature profiles than free laccase have been exhibited by the immobilized laccase. Thermal stability was enhanced to a great extent and operational stability was increased to a certain extent. The shift of kinetic parameters indicated affinity change between enzyme and substrate. Application of the immobilized system in phenol and p-chlorophenol removal was investigated in a batch system. Adsorption effects of the support were responsible for the quick removal rate in the first hour, and up to 78% and 84% of phenol and p-chlorophenol were removed in the end of the reaction, respectively, indicating that the magnetic bimodal mesoporous carbon is a promising carrier for both immobilization of laccase and further application in phenol removal.


► A new laccase immobilized system was built using magnetic bimodal mesoporous carbon.
► Immobilized laccase exhibited excellent adsorption capacity and activity recovery.
► Immobilization improved reusability and resistance to pH and temperature changes.
► Nearly 80% of phenols were removed by the immobilized laccase in 12 h.
► Adsorption effects of the support mainly contributed to the first hour of removal.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 115, July 2012, Pages 21–26
نویسندگان
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