کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
681566 1460028 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel and efficient method for the immobilization of thermolysin using sodium chloride salting-in and consecutive microwave irradiation
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
A novel and efficient method for the immobilization of thermolysin using sodium chloride salting-in and consecutive microwave irradiation
چکیده انگلیسی

Sodium chloride salting-in and microwave irradiation were combined to drive thermolysin molecules into mesoporous support to obtain efficiently immobilized enzyme. When the concentration of sodium chloride was 3 M and microwave power was 40 W, 93.2% of the enzyme was coupled to the support by 3 min, and the maximum specific activity of the immobilized enzyme was 17,925.1 U mg−1. This was a 4.5-fold increase in activity versus enzyme immobilized using conventional techniques, and a 1.6-fold increase versus free enzyme. Additionally, the thermal stability of the immobilized thermolysin was significantly improved. When incubated at 70 °C, there was no reduction in activity by 3.5 h, whereas free thermolysin lost most of its activity by 3 h. Immobilization also protected the thermolysin against organic solvent denaturation. The microwave-assisted immobilization technique, combined with sodium chloride salting-in, could be applied to other sparsely soluble enzymes immobilization because of its simplicity and high efficiency.


► Salting-in and microwave irradiation were combined to immobilize thermolysin.
► The maximum immobilized enzyme activity was 4.5-fold of that using normal method.
► Both the thermal stability and stability against organic solvents were enhanced.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 115, July 2012, Pages 158–163
نویسندگان
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