کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
682242 888978 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High efficient expression of cellobiase gene from Aspergillus niger in the cells of Trichoderma reesei
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
High efficient expression of cellobiase gene from Aspergillus niger in the cells of Trichoderma reesei
چکیده انگلیسی

The cellobiase gene from Aspergillus niger was cloned and connected with the strong promoter Pcbh1 from Trichoderma reesei to construct a recombinant plasmid pHB9 with the hygromycin B resistance marker. The plasmid was transformed into conidia of T. reesei using the modified PEG–CaCl2 method. Main factors effecting the transformation were discussed and about 99–113 transformants/μg DNA could be obtained under optimal conditions. It was found that the molecular mass of the recombinant cellobiase was about 120 kDa by SDS–PAGE analysis. The activity of cellobiase could reach 5.3 IU/ml after 48 h fermentation, which was as high as 106 times compared with that of the host strain. Meanwhile, the filter paper activity of recombinant T. reesei was 1.44-fold of the host strain. Saccharification of corncob residue with the crude enzyme showed that the hydrolysis yield (84.2%) of recombinant T. reesei was 21% higher than that (69.5%) of the host strain.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 102, Issue 6, March 2011, Pages 4568–4572
نویسندگان
, ,