کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
682727 | 888990 | 2010 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immobilization of intracellular carbonyl reductase from Geotrichum candidum for the stereoselective reduction of 1-naphthyl ketone
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Immobilization of intracellular carbonyl reductase from Geotrichum candidum for the stereoselective reduction of 1-naphthyl ketone Immobilization of intracellular carbonyl reductase from Geotrichum candidum for the stereoselective reduction of 1-naphthyl ketone](/preview/png/682727.png)
چکیده انگلیسی
Different cell disintegration methods were used for the liberation of intracellular carbonyl reductase from Geotrichum candidum, in its active form. Solid shear (bead milling) was proved to be the best method for the extraction of the enzyme. Various solid supports were checked for the immobilization of the purified enzyme. Carbonyl reductase was immobilized on silica with an optimized protein loading of 4 mg/g support. Cross-linking with glutaraldehyde rendered the preparation more stable and suitable for use in consecutive batches. Carbonyl reductase of G. candidum immobilized on silica support and cross-linked by glutaraldehyde was found to be highly efficient biocatalyst formulation for the production of S(−)-1-(1′-naphthyl) ethanol.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 101, Issue 6, March 2010, Pages 1581–1586
Journal: Bioresource Technology - Volume 101, Issue 6, March 2010, Pages 1581–1586
نویسندگان
Mani Shankar Bhattacharyya, Amit Singh, U.C. Banerjee,