کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
683573 889002 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Engineering of glycerol utilization pathway for ethanol production by Saccharomyces cerevisiae
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
Engineering of glycerol utilization pathway for ethanol production by Saccharomyces cerevisiae
چکیده انگلیسی

Saccharomyces cerevisiae was metabolically engineered to improve ethanol production from glycerol. High rates of glycerol utilization were achieved by simultaneous overexpression of glycerol dehydrogenase (Gcy) and dihydroxyacetone kinase (Dak), which are the enzymes responsible for the conversion of glycerol to glycolytic intermediate dihydroxyacetone phosphate. As a result, ethanol production in YPH499 (pGcyaDak) was about 2.4-fold higher than wild strain. We have also successfully expressed a glycerol uptake protein (Gup1). The overall ethanol production in strain YPH499 (pGcyaDak, pGupCas) was 3.4-fold more than in wild strain, with about 2.4 g L−1 ethanol produced. These experimental results confirmed our metabolic pathway strategies which improve the production of ethanol.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 101, Issue 11, June 2010, Pages 4157–4161
نویسندگان
, , ,