کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
686508 | 889083 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A highly thermostable and alkaline amylase from a Bacillus sp. PN5
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A highly thermostable and alkaline amylase from a Bacillus sp. PN5 A highly thermostable and alkaline amylase from a Bacillus sp. PN5](/preview/png/686508.png)
چکیده انگلیسی
A highly thermostable alkaline amylase producing Bacillus sp. PN5 was isolated from soil, which yielded 65.23 U mL−1 of amylase in medium containing (%) 0.6 starch, 0.5 peptone and 0.3 yeast extract at 60 °C, pH 7.0 after 60 h of incubation. Maximum amylase activity was at pH 10.0 and 90 °C. The enzyme retained 80% activity after 1 h at pH 10.0. It exhibited 65% activity at 105 °C and had 100% stability in the temperature range between 80 and 100 °C for 1 h. In addition, there was 86.36% stability after 1-h incubation with sodium dodecylsulphate. These properties indicated possible use of this amylase in starch saccharification and detergent formulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 98, Issue 2, January 2007, Pages 260–265
Journal: Bioresource Technology - Volume 98, Issue 2, January 2007, Pages 260–265
نویسندگان
Rajendra Kumar Saxena, Kakoli Dutt, Lata Agarwal, Preeti Nayyar,