Identification and characterization of a novel Old Yellow Enzyme from Bacillus subtilis str.168

• Identifing a new “thermophilic-like” OYEs from Bacillus subtilis str.168.
• Exhibiting high tolerance towards temperature and pH.
• Exhibiting high activities towards maleimide, dimethyl maleate and acyclic ketones.
• The YqiG can catalyze citral to (S)-citronellal with high e.e% (>99%).

We identified and characterized YqiG, a novel Old Yellow Enzyme (OYE) from Bacillus subtilis str.168, as a member of the “thermophilic-like” subfamily. It is most related to XenA from Pseudomonas putida, with 37.9% identity, but it exhibits certain differences in sequence and enzyme properties. The YqiG can reduce various activated alkenes and exhibits high temperature (60 °C for 12 h) and pH stability (pH 4.0 and 9.0 for 12 h), which indicates that it has a great potential for biocatalysis. However, it shows low tolerance toward organic solvents. Also the YqiG shows high activities towards maleimide, dimethyl maleate and acyclic ketones (55.5 U/mg, 6.22 U/mg and 2.97 U/mg, respectively). Besides, The YqiG can catalyze citral to (S)-citronellal with a low rate but high ee% (>99%).

Figure optionsDownload as PowerPoint slide