Using active microfluidic flow focusing to sort particles and cells based on high-resolution volume measurements

- We combine a high-resolution resistive pulse sensor with hydrodynamic flow focusing.
- We quantify sorting purity, throughput and resolution with polystyrene microspheres.
- We size/sort a population of Saccharomyces cerevisiae (yeast) cells.
- We integrate PDMS valves, both for sensitivity enhancement and cell collection.