|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|69457||48770||2016||5 صفحه PDF||سفارش دهید||دانلود رایگان|
• CALB was immobilized on magnetic PUU NPs by interfacial polymerization.
• Immobilization of CALB and the support synthesis were conducted simultaneously.
• Increasing the content of CALB increased the particle diameter.
• Immobilized CALB showed higher esters conversion.
In this work Candida antarctica lipase B (E.C: 22.214.171.124), was immobilized on magnetic poly(urea-urethane) nanoparticles (MNPs-PUU) in a single-step during the interfacial miniemulsion polymerization. Transmission electron microscopy images showed the morphology of synthesized magnetic nanoparticles encapsulated in poly(urea-urethane) nanoparticles and fluorescence microscopy images confirmed the enzyme immobilization onto MNPs-PUU. After the immobilization process, the immobilized enzyme on support (MNPs-PUU) was attracted by an external magnetic field and used as biocatalyst for the synthesis of esters ethyl oleate, geranyl propionate and geranyl oleate. Ester conversions above 85% were obtained for all systems based on the free fatty acids contents measured by titration until pH 11.
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Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 131, September 2016, Pages 31–35