کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
69660 | 48786 | 2015 | 6 صفحه PDF | دانلود رایگان |
• Rhodotorula sp. LSL reduces prochiral arylketones with Prelog stereoselectivity.
• Optimized lyophilized biocatalyst was used at extreme basic pH.
• One-pot procedures were developed to access to enantiopure building blocks.
• Halohydrins, oxiranes and diols were chemodivergently prepared from α-haloarylketones.
Rhodotorula sp. LSL, isolated from a local landfarming was able to catalyze the reduction of prochiral arylketones into sec-alcohols with excellent Prelog stereoselectivity (ee > 99%). The use of resting and lyophilized cells was optimized accessing to an easy-to-use whole cell biocatalyst that efficiently works even under non-sterile conditions, without the addition of external cofactors and using plain water as solvent. The catalyst resistance in alkaline media enabled (chemo)enzymatic one-pot procedures at high pH values. So a simple and efficient methodology was applied to prepare alternatively enantiopure β-halohydrins, terminal diols and epoxides from aromatic α-haloketones.
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Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 114, April 2015, Pages 19–24