Confocal Raman microscopy for mapping phase segregation in individually dried particles composed of lactose and macromolecules

- Confocal Raman microscopy is a powerful tool for internal mapping of particles.
- Phase segregation occurs even during the short drying time of particles.
- Macromolecules are enriched at the surface of the dried particles.
- A macromolecule depleted layer is observed below the surface.
- The macromolecules are segregated from the lactose matrix as a thick surface layer.