Design of an electrochemical biosensing system for xanthine detection and a study on binding interaction of ketoconazole with xanthine oxidase

- XO was covalently immobilized on poly(glycidyl methacrylate-co-vinylferrocene).
- Physicochemical parameters was determined by monitoring response current at 0.6 V.
- Ktc-XO interaction was studied by spectrofluorometric and electrochemical methods.
- Binding interaction of Ktc with XO was confirmed by SDS-PAGE and native-PAGE analysis.