کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
69810 | 48794 | 2013 | 7 صفحه PDF | دانلود رایگان |

• As a biocatalyst we employed the strains which are commonly used in food industry.
• Found an effective way to obtain dihydrochalcones (sweet taste).
• Tested biocatalysts are characterized with a very high substrate specificity.
• Changes in the substrate structures may activate different enzymes of a catalyst used.
Biotransformations of two substrates: chalcone (1) and 2′-hydroxychalcone (4) were carried out using four yeast strains and five filamentous fungi cultures. Substrate 1 was effectively hydrogenated in all of tested yeast cultures (80–99% of substrate conversion after 1 h of biotransformation) affording dihydrochalcone 2. In the cultures of filamentous fungi the reaction was much slower, however, Chaetomium sp. gave product 2 in 97% yield. After 12 h of incubation a reduction of dihydrochalcone 2 to alcohol 3 was additionally observed. After 3 days of biotransformation in the culture of Rhodotorula rubra product (S)-3 was obtained with 75% ee (enantiomeric excess) and 99% of conversion. Also after a 3-day biotransformation using the strain Fusarium culmorum product (R)-3 was obtained with 98% ee and 97% of conversion. In most of the tested strains a change in enantiomeric excess of compound 3 during the biotransformation process was noticed. In the culture of Rhodotorula glutinis after 3 h of transformation alcohol (R)-3 was formed with 47% ee and 31% of substrate conversion, whereas after 6 days the (S)-3 enantiomer was obtained with 99% ee and 91% of conversion. In the case of 2′-hydroxychalcone (4), the hydrogenation proceeded much slower and led to 2′-hydroxydihydrochalcone (5) – in the culture of Yarrowia lipolytica 97% of conversion was observed after 3 days. In all cultures of the tested strains no products of 2′-hydroxydihydrochalcone reduction were detected.
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Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 98, 30 December 2013, Pages 55–61