کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
69845 48796 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enzymatic preparation of a natural sweetener rubusoside from specific hydrolysis of stevioside with β-galactosidase from Aspergillus sp.
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی کاتالیزور
پیش نمایش صفحه اول مقاله
Enzymatic preparation of a natural sweetener rubusoside from specific hydrolysis of stevioside with β-galactosidase from Aspergillus sp.
چکیده انگلیسی

Rubusoside is a precious bioactive sweetener which mainly exists in Chinese sweet tea plant (Rubus suavissimus S. Lee), while stevioside is an abundant natural sweetener with bitter aftertaste. In this work, a β-galactosidase from Aspergillus sp. presented specific hydrolytic activity on β-1,2 glucosidic linkage of stevioside, and converting stevioside to rubusoside. The hydrolytic activity was weak on the natural stevioside analogs, which makes the process a promising approach to produce rubusoside and utilize stevioside. The highest steviosides conversion was 98.3%, accompanying with a rubusoside yield of 91.4%.

Rubusoside is a precious bioactive natural sweetener, while stevioside is an abundant sweetener with bitter aftertaste. A β-galactosidase from Aspergillus sp. was employed to specifically catalyze the hydrolysis of β-1,2 glucosidic linkage of stevioside, which provides a new route to transfer stevioside to rubusoside.Figure optionsDownload as PowerPoint slideHighlights
► A rare natural sweetener rubusoside was synthesized from abundant stevioside.
► The β-galactosidase presented specific hydrolytic activity on stevioside.
► The highest rubusoside yield was 91.4% with a stevioside conversion of 98.3%.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 82, October 2012, Pages 12–17
نویسندگان
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