کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
69845 | 48796 | 2012 | 6 صفحه PDF | دانلود رایگان |

Rubusoside is a precious bioactive sweetener which mainly exists in Chinese sweet tea plant (Rubus suavissimus S. Lee), while stevioside is an abundant natural sweetener with bitter aftertaste. In this work, a β-galactosidase from Aspergillus sp. presented specific hydrolytic activity on β-1,2 glucosidic linkage of stevioside, and converting stevioside to rubusoside. The hydrolytic activity was weak on the natural stevioside analogs, which makes the process a promising approach to produce rubusoside and utilize stevioside. The highest steviosides conversion was 98.3%, accompanying with a rubusoside yield of 91.4%.
Rubusoside is a precious bioactive natural sweetener, while stevioside is an abundant sweetener with bitter aftertaste. A β-galactosidase from Aspergillus sp. was employed to specifically catalyze the hydrolysis of β-1,2 glucosidic linkage of stevioside, which provides a new route to transfer stevioside to rubusoside.Figure optionsDownload as PowerPoint slideHighlights
► A rare natural sweetener rubusoside was synthesized from abundant stevioside.
► The β-galactosidase presented specific hydrolytic activity on stevioside.
► The highest rubusoside yield was 91.4% with a stevioside conversion of 98.3%.
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 82, October 2012, Pages 12–17