کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
69861 | 48796 | 2012 | 6 صفحه PDF | دانلود رایگان |
In this work, a reliable protocol was designed to rapidly express and purify a rat diamine oxidase in Escherichia coli as a useful alternative to enzyme isolated from animal sources. The cDNA encoding for rat diamine oxidase was overexpressed in an Origami2(DE3) E. coli strain and, by employing a rapid purification protocol in which the hexahistidine tag was added at the C-terminal end of the enzyme, the recombinant oxidase could be purified in a single step on a Ni-NTA column at >95% purity. The enzyme was active but was largely produced in an immature quinone form: Cu2+ ions stimulated further activation/maturation. This expression and purification procedure offers an easy and rapid means of producing recombinant rat diamine oxidase from an animal-free source and represents a useful tool to boost biotechnological application of this enzyme.
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► Rat diamine oxidase (DAO) was overexpressed in E. coli.
► The purified recombinant DAO (2 mg pure protein/L broth) was ≥95% pure.
► The recombinant DAO was active but was largely produced in an immature quinone form.
► Cu2+ ions significantly stimulated further activation/maturation.
► This procedure produces recombinant rat diamine oxidase from an animal-free source.
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 82, October 2012, Pages 115–120