کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
69880 48799 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
FSAB: A new fructose-6-phosphate aldolase from Escherichia coli. Cloning, over-expression and comparative kinetic characterization with FSAA
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی کاتالیزور
پیش نمایش صفحه اول مقاله
FSAB: A new fructose-6-phosphate aldolase from Escherichia coli. Cloning, over-expression and comparative kinetic characterization with FSAA
چکیده انگلیسی

Fructose-6-phosphate aldolase B (FSAB) from Escherichia coli was successfully over-expressed as His-tagged recombinant protein. A decameric protein was observed as for FSAA. Unlike FSAA, FSAB is not thermally stable at temperatures higher than 60 °C. The 70% identity between the two aldolases has allowed the generation of a 3D structure which has shown a high similarity of the two active sites. Full kinetic studies towards several substrates have revealed that FSAB catalytic activity is very close to FSAA activity, corroborated by the similarity of their active sites. FSAB has been able to react with three known donors (dihydroxyacetone, hydroxyacetone and glycolaldehyde) but always slightly slower than FSAA.

Figure optionsDownload as PowerPoint slideHighlights
► FSAB overexpressed as His-tagged recombinant protein is studied.
► A decameric form has been determined like for FSAA.
► Kinetic parameters towards several substrates were determined.
► The same substrates tolerance as for FSAA has been demonstrated.
► 3D structure model was generated.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 84, December 2012, Pages 9–14
نویسندگان
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