کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
69904 48799 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Immobilization of marine fungi on silica gel, silica xerogel and chitosan for biocatalytic reduction of ketones
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی کاتالیزور
پیش نمایش صفحه اول مقاله
Immobilization of marine fungi on silica gel, silica xerogel and chitosan for biocatalytic reduction of ketones
چکیده انگلیسی

The scanning electron microscopy (SEM) analysis showed that whole living hyphal of marine fungi Aspergillus sclerotiorum CBMAI 849 and Penicillium citrinum CBMAI 1186 were immobilized on support matrices of silica gel, silica xerogel and/or chitosan. P. citrinum immobilized on chitosan catalyzed the quantitative reduction of 1-(4-methoxyphenyl)-ethanone (1) to the enantiomer (S)-1-(4-methoxyphenyl)-ethanol (3b), with excellent enantioselectivity (ee > 99%, yield = 95%). Interestingly, ketone 1 was reduced with moderate selectivity and conversion to alcohol 3b (ee = 69%, c 40%) by the free mycelium of P. citrinum. This free mycelium of P. citrinum catalyzed the production of the (R)-alcohol 3a, the antipode of the alcohol produced by the immobilized cells. P. citrinum immobilized on chitosan also catalyzed the bioreduction of 2-chloro-1-phenylethanone (2) to 2-chloro-1-phenylethanol (4a,b), but in this case without optical selectivity. These results showed that biocatalytic reduction of ketones by immobilization hyphal of marine fungi depends on the xenobiotic substrate and the support matrix used.

Whole living cells of P. citrinum immobilized on chitosan for reduction of ketones.Figure optionsDownload as PowerPoint slideHighlights
► Immobilization of whole living cells marine fungi on support matrices of silica gel, silica xerogel and chitosan.
► Penicilllum citrinum immobilized on chitosan catalyzed efficiently the reduction of ketones.
► Scanning electron microscopy showed a strong interaction between chitosan and the mycelium of P. citrinum.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 84, December 2012, Pages 160–165
نویسندگان
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