Baohuoside I production through enzyme hydrolysis and parameter optimization by using response surface and subset selection

A rapid and efficient baohuoside I preparation method was established. A uniform design coupled with subset selection was employed to determine pH, reaction time, and temperature parameters, as well as dextranase hydrolysis efficiency. Hydrolysis parameters were optimized using response surface and subset selection. Our results showed that pH plays an important role in the hydrolysis reaction within a relatively narrow range (pH 4–7). Temperature was the secondary factor, which was positively correlated with conversion rate. A 3-h reaction time was sufficient. Finally, a relatively good hydrolysis parameters were found, and their effectiveness was verified.

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► High purity Baohuoside I was prepared by dextranase hydrolysis.
► RSM and subset selection were used to optimize reaction parameters.
► Parameters for industrial production were also established.
► This method is suitable for experimental and industrial scale for acceptable purity.