One-pot three-step continuous enzymatic synthesis of 5-fluoro-5-deoxy-d-ribose

The one-pot three-step continuous enzymatic synthesis of 5-fluoro-5-deoxy-d-ribose (5-FDR) from ATP and l-methionine using S-adenosyl-l-methionine synthase (MetK), fluorinase and methylthioadenosine nucleosidase (MtnN) in the presence of fluoride ion, was described. Especially, for the purpose of the sustainable development of fluorine chemistry, the reuse of fluoride ion generated from BF4 ionic liquids and/or the biodegradation of BTF in the one-pot synthetic process to 5-FDR, was described.

Figure optionsDownload as PowerPoint slideHighlights
► We examine the one-pot 3-step continuous enzymatic synthesis of 5-fluoro-5-deoxy-d-ribose (5-FDR) from ATP and L-methionine using MetK, fluorinase and MtnN in the presence of fluoride ion.
► We examine the one-pot 3-step continuous enzymatic route to 5-FDR using a fluoride ion generated from the degradatuion of BF4 ionic liquid and/or BTF.