کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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70094 | 48810 | 2013 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Microbial stereospecific reduction of 3-quinuclidinone with newly isolated Nocardia sp. and Rhodococcus erythropolis Microbial stereospecific reduction of 3-quinuclidinone with newly isolated Nocardia sp. and Rhodococcus erythropolis](/preview/png/70094.png)
Two bacterium strains, Nocardia sp. WY1202 and Rhodococcus erythropolis WY1406, were isolated from soil samples. They catalyzed the asymmetric reduction of 3-quinuclidinone to give enantiomeric pure (R)- and (S)-3-quinuclidinol, respectively. The optimal temperatures for the bioreduction by Nocardia sp. and R. erythropolis were 30 °C and 37 °C, respectively, while both strains showed highest activity at pH 8.0. Without external addition of expensive NADH or NADPH, (R)-3-quinuclidinol and (S)-3-quinuclidinol were obtained with 93% and 92% isolated yield and >99% enantiomeric excess. As such, microbial reduction by Nocardia sp. WY1202 or R. erythropolis WY1406 offers a new stereospecific approach to both antipodes of 3-quinuclidinol of pharmaceutical importance.
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► Two bacterial strains, Nocardia sp. WY1202 and Rhodococcus erythropolis WY1406, were isolated.
► They catalyzed the enantioselective reduction of 3-quinuclidinone to give enantiomeric pure (R)- and (S)-3-quinuclidinol, respectively.
► Without external NADH or NADPH, (R)- and (S)-3-quinuclidinol (>99% ee) were obtained with 93% and 92% isolated yield, respectively.
► This study offers a new stereospecific approach to both antipodes of 3-quinuclidinol.
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 88, April 2013, Pages 14–19