کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
70125 48812 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Using ionic liquid cosolvents to improve enzymatic synthesis of arylalkyl β-d-glucopyranosides
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی کاتالیزور
پیش نمایش صفحه اول مقاله
Using ionic liquid cosolvents to improve enzymatic synthesis of arylalkyl β-d-glucopyranosides
چکیده انگلیسی

Enzymatic synthesis of various arylalkyl β-d-glucopyranosides catalyzed by prune (Prunus domestica) seed meal via reverse hydrolysis in the mixture of organic solvent, ionic liquid (IL) and phosphate buffer was described. Among four hydrophilic organic solvents tested, ethylene glycol diacetate (EGDA) was found to be the most suitable for enzymatic synthesis of salidroside, a bioactive compound of commercial interest, from d-glucose and tyrosol. The effects of the nature of ionic liquids and their contents on the enzymatic glucosylation were studied. The addition of a suitable amount of ILs including denaturing ones was favorable to shift the reaction equilibrium toward the synthesis, thus improving the yields. Among the examined ILs, the novel IL [BMIm]I proved to be the best. And this IL was applied as the solvent in biocatalysis for the first time. The yields were found to be enhanced between 0.2-fold and 0.5-fold after the addition of 10% (v/v) [BMIm]I. In 10% (v/v) [BMIm]I-containing system, the desired arylalkyl β-d-glucopyranosides were synthesized with 15–28% yields, among which salidroside was obtained with a yield of 22%.

Figure optionsDownload as PowerPoint slideHighlights
► Ethylene glycol diacetate, a green and nontoxic organic solvent, proved to be the best for enzymatic synthesis of glycosides.
► The ionic liquid [BMIm]I was applied as the solvent in biocatalysis for the first time.
► The yields were enhanced between 0.2-fold and 0.5-fold by adding 10% (v/v) [BMIm]I.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 74, Issues 1–2, January 2012, Pages 24–28
نویسندگان
, , ,