کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
70163 | 48813 | 2013 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The open lid conformation of the lipase is explored in the compressed gas: New insights from molecular dynamic simulation The open lid conformation of the lipase is explored in the compressed gas: New insights from molecular dynamic simulation](/preview/png/70163.png)
The advantages of enzymatic reactions in compressed gases such as supercritical CO2 are limited due to the enzyme inactivation. But recent experimental observations reported the high activity of enzymes such as lipases in compressed propane. But there are no clear reasons at the molecular levels for such behavior. In this work using molecular dynamic simulation, we showed for the first time the possibility of interfacial activation of lipases in a compressed gas. The analysis showed that in compressed propane the lid of the lipase was opened and so the active conformation of the enzyme was resulted. Moreover it is found that in the compressed propane, similar to the aqueous solution, the enzyme has native conformation.
Figure optionsDownload as PowerPoint slideHighlights
► For the first time interfacial activation of the lipase is reported in a compressed gas.
► In water the lid shifted from open to close but in compressed propane reverse phenomenon was observed.
► The enzyme has stable conformation in compressed propane.
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 87, March 2013, Pages 135–138