Enzymatic preparation of melibiose and alkyl β-d-fructofuranosides by commercial lactase

Lactozyme 3000L was found to possess β-fructofuranosidase activity and catalyzed hydrolysis of raffinose to melibiose and formation of alkyl β-d-fructofuranosides from sucrose. The total chemical yield of melibiose was 95.5%, yields of alkyl β-d-fructofuranosides ranged from 5.2 to 20.2% depending on the structure of the acceptor. Alcohols larger than butanol were not glycosylated.

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► Lactozyme 3000L as a source of β-fructofuranosidase.
► Catalyst for raffinose hydrolysis and transfructosidations.
► Alcohols longer than butanol are not fructosylated.