کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
70242 | 48818 | 2012 | 8 صفحه PDF | دانلود رایگان |
Biocatalytic synthesis is now well established amongst catalytic methodologies as an extremely useful approach for the industrial synthesis of high-value compounds, due to its many advantages such as high reaction specificity and selectivity. However, engineering a biocatalytic process can be complex and time-consuming. This paper presents a modular microfluidic reactor and in-line filtration system for the rapid and small-scale evaluation of biocatalytic reactions. The system combines a substrate with a biocatalyst in free solution, incubates the two components until full conversion to product has been achieved, before extracting the product. The system has been applied to the transketolase-catalysed reaction of hydroxypyruvate (HPA) and glycolaldehyde (GA) to l-erythrulose, demonstrating complete conversion of substrate to product, complete retention of the enzyme and an overall yield of approximately 65%. The complete conversion of HPA and propanal to (3S)-1,3-dihydroxypentan-2-one with a mutant transketolase further demonstrated the applicability of the microfluidic system for organic synthesis.
Figure optionsDownload as PowerPoint slideHighlights
► We present integration of up- and downstream microfluidic bioprocess development.
► The system has been used for rapid small-scale evaluation of biocatalytic reactions.
► Complete conversion of substrate to product was achieved in the microreactor.
► The downstream filter unit allows the integration of any membrane type.
► Results from the microfluidic system are scalable.
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 77, May 2012, Pages 1–8