کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
703140 | 891127 | 2007 | 4 صفحه PDF | دانلود رایگان |

Double stranded deoxyribonucleic acid (ds-DNA) layers, bonded to hydrogen terminated polycrystalline diamond, are characterized by scanning electron (SEM), fluorescence (FM), and atomic force microscopy (AFM). DNA grafting has been achieved using photochemical bonding of ω-unsaturated 10-amino-dec-1-ene molecules. SEM detects local variations of electron affinities on polycrystalline diamond, revealing distinct grain structures. FM applied on fluorescence labeled ds-DNA show laterally varying intensities of typically 20%, which resembles also grain structure as detected by SEM. Contact and tapping-mode AFM characterization reveal a tilted DNA bonding to diamond and a dense layer formation which gives rise to smoothening of surface properties. The lateral density variation of DNA is attributed to local variations of the photo-electron emission efficiency which affects the photochemical attachment chemistry of amine-linker molecules to diamond.
Journal: Diamond and Related Materials - Volume 16, Issue 8, August 2007, Pages 1648–1651