Lipase-catalyzed synthesis and characterization of novel lipidyl-cyclodextrins in solvent free medium

The lipase-catalyzed amidation reaction between fatty acyl donors and mono-6-amino-permethylated β-cyclodextrin (MBCD-NH2) was achieved with high yield in solvent free medium. The ability of Mucor miehie and Candida cylindracea lipases, free or immobilized, to catalyze amidation reaction was shown. Products were purified and characterized. To optimize the reaction, some reaction conditions were assessed like temperature, type of shaking and amount of enzyme, on a model amidation reaction between butyl caprylate and MBCD-NH2. Among the biocatalysts tested, Lipozyme was found to be the most active, requiring low loading, being robust at 70 °C even under magnetic agitation.