کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7067386 | 1459884 | 2018 | 36 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mixing effects on the kinetics and the dynamics of two-phase enzymatic hydrolysis of hemicellulose for biofuel production
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
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چکیده انگلیسی
This work uses a coupled experimental and modeling approach to explore the effects of macro- and micro-mixing on the kinetics and the dynamics of two-phase enzymatic hydrolysis of hemicellulose. Reactor mixing does not alter the non-competitive nature of product inhibition in hemicellulose hydrolysis by endoxylanase, but produces stronger inhibition that reduces the soluble sugar yield by 8-14.5%, as the mixing speed increases from 0 to 200â¯rpm. The kinetic constants (Km, Vmax, Kx) assume mass-transfer disguised values at 0-200â¯rpm. An optimal mixing strategy, comprising of 55-70â¯min of initial rapid convective macromixing followed by diffusive micromixing (without any macromixing) for the rest of the hydrolysis, increases xylose and reducing sugar yields by 6.3-8% and 13-20%, respectively, over continuous mixing at 200â¯rpm, for 1-5â¯mg/ml substrate loading at an optimum enzyme to substrate ratio of 1:20, with an energy saving of 94-96% over 24â¯h.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 259, July 2018, Pages 276-285
Journal: Bioresource Technology - Volume 259, July 2018, Pages 276-285
نویسندگان
Sajal Kanti Dutta, Saikat Chakraborty,