Ionic liquid co-lyophilized enzyme for biocatalysis in organic solvent: Remarkably enhanced activity and enantioselectivity

The room temperature solid-phase ionic liquid (RTSPIL) co-lyophilized enzyme exhibited markedly enhanced activity in organic solvent. The enzyme co-lyophilized with a dodecyl-imidazolium salt was 660-fold more active compared to its RTSPIL-free counterpart. The activity enhancement by RTSPILs was mainly attributable to the reduced particle sizes and improved dispersion of enzymes suspended in organic solvent. Also, the RTSPIL co-lyophilized enzyme displayed significantly enhanced enantioselectivity. Its enantioselectivity was 2.5-fold higher than that of its RTSPIL-free counterpart.

Ionic liquid co-lyophilized enzyme is described as a useful catalyst for biocatalysis in organic solvent. The some room-temperature-solid-phase ionic liquids were synthesized and used as the lyoprotectant material for the lyophilization of enzyme.Figure optionsDownload as PowerPoint slideResearch highlights▶ The room temperature solid-phase ionic liquids (RTSPILs) co-lyophilized enzyme exhibited markedly enhanced reactivity for biocatalysis in organic solvent. ▶ The enantioselectivity of RTSPIL co-lyophilized enzyme presents the enhanced enantioselectivity. ▶ The enhanced activities by RTSPILs are mainly attributable to the size affection of enzyme catalyst after co-lyophilization with RTSPIL.