کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7068475 | 1459891 | 2018 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immobilization of Aspergillus niger cellulase on multiwall carbon nanotubes for cellulose hydrolysis
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Immobilization of Aspergillus niger cellulase on multiwall carbon nanotubes for cellulose hydrolysis Immobilization of Aspergillus niger cellulase on multiwall carbon nanotubes for cellulose hydrolysis](/preview/png/7068475.png)
چکیده انگلیسی
In present study, Aspergillus niger cellulase was immobilized onto functionalized multiwalled carbon nanotubes (MWCNTs) via carbodiimide coupling. MWCNTs offer unique advantages including enhanced electronics properties, a large edge to basal plane ratio, rapid electrode kinetics and it's possess higher tensile strength properties due to their structural arrangements. The immobilization was confirmed by FTIR (Fourier transform infrared spectroscopy) and SEM (scanning electron microscope). The bionanoconjugates prepared under optimized condition retained 85% activity with improved pH and thermal stability. The t1/2 of immobilized cellulase at 70â¯Â°C was four fold higher than free enzyme. The Km value indicates that affinity of bionanoconjugates towards substrate has increased by two times. The preparation could be reused ten times without much loss in enzyme activity. The enhanced catalytic efficiency, stability and reusability makes it useful for efficient cellulose hydrolysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 252, March 2018, Pages 72-75
Journal: Bioresource Technology - Volume 252, March 2018, Pages 72-75
نویسندگان
Razi Ahmad, Sunil Kumar Khare,