Effect of seed sludge on nitrogen removal in a novel upflow microaerobic sludge reactor for treating piggery wastewater

Anaerobic activated sludge (AnaS) and aerobic activated sludge (AerS) were used to start up a novel upflow microaerobic sludge reactor (UMSR), respectively, and the nitrogen removal in the two reactors were evaluated when treating low C/N ratio manure-free piggery wastewater with a COD/TN ration of about 0.85. With the same hydraulic retention time 8 h and TN loading rate (NLR) 0.42 kg/(m3 d), the UMSR (R2) inoculated with AerS could reach its steady state earlier and obtained a better TN removal than that in the UMSR (R1) inoculated with AnaS. However, the accumulated AnaS made R1 show a better capability in bearing shock load and demonstrated an excellent NH4+-N and TN removal with a NLR as high as 1.07 kg/(m3 d). Microbial community structure of the accumulated AerS and AnaS were observable different. The decreased proportion of nitrifiers restricted the ammonium oxidation in R2, and resulting in a decrease in TN removal.