کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7082311 1459999 2013 18 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High activity cellulase production by recombinant Trichoderma reesei ZU-02 with the enhanced cellobiohydrolase production
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
High activity cellulase production by recombinant Trichoderma reesei ZU-02 with the enhanced cellobiohydrolase production
چکیده انگلیسی
The cbh1 strong promoter was employed to over-express the cbh2 gene for enhancing cellobiohydrolase (CBH) production in Trichoderma reesei because CBH II component has higher specific activity than CBH I and is an important component in cellulase. The recombinant plasmid pCAMBIA1300-hph-PsCT containing strong expression cassette was constructed and transformed into T. reesei via optimized Agrobacterium-mediated transformation, producing 324 positive T. reesei transformants for the two steps of screening. Ten fast-growing T. reesei transformants were selected, amongst which C10 was found to have the highest filter paper activity 28.92 ± 2.45 IU/mL, 4.3-fold higher than that of ZU-02, 6.71 ± 0.79 IU/mL. C10 also has the highest cellobiohydrolase activity 122.44 ± 7.42 U/mL, 5.4 times higher than that of ZU-02, 22.49 ± 2.27 U/mL. The cellulase from C10 performed better (93.06 ± 2.83%) than the one from ZU-02 in enzymatic hydrolysis because the exo-exo-synergism played a role.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 144, September 2013, Pages 693-697
نویسندگان
, ,