Optimizing the enzyme loading and incubation time in enzymatic hydrolysis of lignocellulosic substrates

► A simple model gives estimated costs of lignocellulosic sugars. ► Variables include enzyme loading and incubation time. ► Those variables can be optimized for lowest sugar cost. ► Optimized values can be expressed in terms of three unit costs. ► Steam-exploded pine feedstock provided data for a worked example.