Sequential accumulation of starch and lipid induced by sulfur deficiency in Chlorella and Parachlorella species

► Starch and lipid accumulation occurred in 4 Chlorellaceae spp. under sulfur deficient conditions. ► Lipids accumulated subsequent to a reduction in stored starch. ► A change in illumination cycle is sufficient for Chlorella lobophora to induce lipid accumulation. ► Fatty acid compositions were altered in Chlorella lobophora and Parachlorella kessleri by sulfur depletion.