Effect of ionic liquid weight ratio on pretreatment of bamboo powder prior to enzymatic saccharification

- Biomass was pretreated with different ionic liquid (IL)/biomass ratios (0-10 g/g).
- Cholinium IL (less expensive and less toxic than conventional IL) was used.
- An IL/biomass ratio of 3 g/g was critical for sufficient biomass pretreatment.
- At an IL/biomass ratio of 3 g/g, biomass was not liquid but solid.
- The solid-state biomass pretreatment using IL can reduce cost and waste water.