Hydrolytic activities of extracellular enzymes in thermophilic and mesophilic anaerobic sequencing-batch reactors treating organic fractions of municipal solid wastes

► Hydrolytic enzyme activities were quantified for ASBRs treating real OFMSW. ► High-rate protein degradation is associated with enhanced performance of TASBR. ► TASBR provides a synergy of thermophilic digestion and sequencing-batch operation. ► Extracellular enzyme activities of thermophiles evidence rapid hydrolysis of OFMSW.