Enzyme-assisted extraction of lipids directly from the culture of the oleaginous yeast Rhodosporidium toruloides

► Enzyme-assisted extraction (EAE) of lipids from the culture of the oleaginous yeast Rhodosporidium toruloides was developed. ► The cell wall lytic enzyme plMAN5C was key to the EAE process. ► The process was optimized with respect to pre-treatment step and extraction solvent. ► Up to 96.6% of the total lipids was extracted from R. toruloides cells at room temperature and atmospheric pressure.