The complete enzymatic saccharification of agarose and its application to simultaneous saccharification and fermentation of agarose for ethanol production

► We developed the complete enzymatic saccharification process for agarose. ► The process is divided into chemical liquefaction and enzymatic saccharification. ► Acectic acid was used as an acid catalyst in the chemcial liquefaction. ► Multiple agarases and a hydrolase were employed in the enzymatic process. ► The process was evaluated by the SSF of agarose for bioethanol production.