Statistical optimization of microalgae Pavlova lutheri cultivation conditions and its fermentation conditions by yeast, Candida rugopelliculosa

► A Sequential strategy based design was applied to optimize the microalgae, Pavlova lutheri mass culture conditions and fermentation conditions. ► Microalgae, Pavlova lutheri is fermented by proteolytic yeast Candidia rugopelliculosa to obtain small peptide chains. ► The optimization of culture and fermentation conditions leads to effective purification of a peptide with hydroxyl radical scavenging activity.