Cyclic, alternating methane and nitrogen limitation increases PHB production in a methanotrophic community

► Three sequencing batch reactors were tested for the selection of PHB production in methanotrophs. ► Repeated nitrogen limitation decreased PHB production in a mixed methanotrophic community. ► Alternating nitrogen PHB content in a mixed methanotrophic community. ► A simulated feast-famine created conditions favorable for PHB production. ► Selection pressures led to dominance by a single strain, Methylocystis parvus OBBP.