کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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71228 | 48888 | 2006 | 5 صفحه PDF | دانلود رایگان |

In this study the effect of the water concentration on a crystallized enzyme of Candida antarctica lipase B (ChiroCLEC™-CAB) in supercritical carbon dioxide (scCO2) is studied. The model reaction used is the enantioselective esterification of racemic 1-phenyl ethanol with vinyl acetate; the reaction is performed in scCO2 at 40 °C and 90 bar in batch and in continuous operation. Kinetic parameters have been derived from continuous experiments, leading to a catalytic turnover number of 0.95 s−1. The optimum activity is reached at low water concentrations (0.05 g L−1). At lower concentrations, CO2 is stripping water from the enzyme leading to deactivation. However, adding a small amount of water to the substrates can reverse this deactivation and the enzyme activity is restored.
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 39, Issues 1–4, 2 May 2006, Pages 112–116