کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
71236 48888 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Distribution of hydantoinase activity in bacterial isolates from geographically distinct environmental sources
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی کاتالیزور
پیش نمایش صفحه اول مقاله
Distribution of hydantoinase activity in bacterial isolates from geographically distinct environmental sources
چکیده انگلیسی

Hydantoin cleaving bacterial isolates were recovered from terrestrial soil samples originating from different geographic sources (Antarctica, South Africa and China) using culture-based screening methods (selective agar plates and shake flask cultures supplemented with hydantoins). Thirty-two bacterial isolates possessing the capability to transform the model substrates benzylhydantoin and dihydrouracil to the corresponding N-carbamoyl-amino acids were successfully cultured. Amplification and sequencing of the 16S rDNA revealed that the isolates belonged to the genera Arthrobacter, Burkholderia, Bacillus, Delftia, Enterobacter, Flavobacterium, Ochrobactrum, Pseudomonas and Stenotrophomonas, with one isolate assigned to the family Microbacteriacae. We have shown that microorganisms with hydantoinase activity are: (i) distributed in various geographically distinct environmental habitats, (ii) distributed worldwide and (iii) found in certain bacterial genera. Furthermore, we have demonstrated the presence of hydantoinase activity in genera in which hydantoinase activity has not previously been reported.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Catalysis B: Enzymatic - Volume 39, Issues 1–4, 2 May 2006, Pages 160–165
نویسندگان
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