کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230333 | 1470946 | 2016 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A fluorescence enhancement probe based on BODIPY for the discrimination of cysteine from homocysteine and glutathione
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Herein, a fluorescent probe BODIPY-based glyoxal hydrazone (BODIPY-GH) (1) for cysteine based on inhibiting of intramolecular charge transfer (ICT) quenching process upon reaction with the unsaturated aldehyde has been synthesized, which exhibits longer excitation wavelength, selective and sensitive colorimetric and fluorimetric response toward cysteine in natural media. The probe shows highly selectivity towards cysteine over homocysteine and glutathione as well as other amino acids with a significant fluorescence enhancement response within 15Â min In the presence of 50 equiv. of homocysteine, the emission increased slightly within 15Â min and completed in 2.5Â h to reach its maximum intensity. Therefore, the discrimination of cysteine from homocysteine and glutathione can be achieved through detection of probe 1. It shows low cytotoxicity and excellent membrane permeability toward living cells, which was successfully applied to detect and image intracellular cysteine effectively by confocal fluorescence imaging.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 85, 15 November 2016, Pages 178-183
Journal: Biosensors and Bioelectronics - Volume 85, 15 November 2016, Pages 178-183
نویسندگان
Deyan Gong, Yuejun Tian, Chengduan Yang, Anam Iqbal, Zhiping Wang, Weisheng Liu, Wenwu Qin, Xiangtao Zhu, Huichen Guo,