کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230389 | 1470947 | 2016 | 24 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biosensor for label-free DNA quantification based on functionalized LPGs
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Biosensor for label-free DNA quantification based on functionalized LPGs Biosensor for label-free DNA quantification based on functionalized LPGs](/preview/png/7230389.png)
چکیده انگلیسی
A label-free fiber optic biosensor based on a long period grating (LPG) and a basic optical interrogation scheme using off the shelf components is used for the detection of in-situ DNA hybridization. A new methodology is proposed for the determination of the spectral position of the LPG mode resonance. The experimental limit of detection obtained for the DNA was 62±2 nM and the limit of quantification was 209±7 nM. The sample specificity was experimentally demonstrated using DNA targets with different base mismatches relatively to the probe and was found that the system has a single base mismatch selectivity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 84, 15 October 2016, Pages 30-36
Journal: Biosensors and Bioelectronics - Volume 84, 15 October 2016, Pages 30-36
نویسندگان
Helena M.R. Gonçalves, Luis Moreira, Leonor Pereira, Pedro Jorge, Carlos Gouveia, Paula Martins-Lopes, José R.A. Fernandes,