کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230471 | 1470946 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Electrochemical immunosensor constructed using TiO2 nanotubes as immobilization scaffold and tracing tag
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Electrochemical immunosensor constructed using TiO2 nanotubes as immobilization scaffold and tracing tag Electrochemical immunosensor constructed using TiO2 nanotubes as immobilization scaffold and tracing tag](/preview/png/7230471.png)
چکیده انگلیسی
A ternary TiO2 nanotube (TNT) composite and a signal antibody and horseradish peroxidase (HRP) functionalized TNT were designed as an electrode scaffold for immobilization of high quantity of capture antibody and a tracing tag for immunosensing, respectively. The polyaniline (PANI) was coated on TNTs by chemical oxidative polymerization, and gold nanoparticles were deposited on TNT-PANI with a routine chemical reduction. Various techniques including scanning electron microscopy, energy dispersive X-ray, transmission electron microscope, X-ray diffraction, Fourier transform infrared spectra, X-ray photoelectron spectra, impedance and electrochemical techniques were used to characterize the nano-materials. Using bis(sulfosuccinimidyl) suberate as amino cross-linker, the TNT composite could be further functionalized with protein Gâ² for oriented immobilization of capture antibody on electrode surface. Upon sandwich-type immunoreaction, the signal antibody on the tracing tag was quantitatively captured on the surface to generate sensitive electrochemical response with a H2O2 mediated HRP catalytic reaction. With α-fetoprotein as an analyte model, the immunosensor showed a linear range of 0.01-350 ng mLâ1 with a detection limit of 1.5 pg mLâ1. The accelerated electron transfer by the ternary composite, oriented immobilization of capture antibody and high loading of HRP on the TNT tracing tag greatly amplified the electrochemical signal, and led to the superior performance of the immunoassay.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 85, 15 November 2016, Pages 698-706
Journal: Biosensors and Bioelectronics - Volume 85, 15 November 2016, Pages 698-706
نویسندگان
Xiaohe Huo, Peipei Liu, Jie Zhu, Xiaoqiang Liu, Huangxian Ju,