کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7230729 | 1470950 | 2016 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bacteria counting method based on polyaniline/bacteria thin film
ترجمه فارسی عنوان
روش شمارش باکتری بر اساس فیلم نازک پلانیلین / باکتری
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کلمات کلیدی
پلی آنیلین، شمارش باکتری ها، فیلم نازک،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A simple and rapid bacteria counting method based on polyaniline (PANI)/bacteria thin film was proposed. Since the negative effects of immobilized bacteria on the deposition of PANI on glass carbon electrode (GCE), PANI/bacteria thin films containing decreased amount of PANI would be obtained when increasing the bacteria concentration. The prepared PANI/bacteria film was characterized with cyclic voltammetry (CV) technique to provide quantitative index for the determination of the bacteria count, and electrochemical impedance spectroscopy (EIS) was also performed to further investigate the difference in the PANI/bacteria films. Good linear relationship of the peak currents of the CVs and the log total count of bacteria (Bacillus subtilis) could be established using the equation Y=â30.413X+272.560 (R2=0.982) over the range of 5.3Ã104 to 5.3Ã108Â CFUÂ mLâ1, which also showed acceptable stability, reproducibility and switchable ability. The proposed method was feasible for simple and rapid counting of bacteria.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 81, 15 July 2016, Pages 75-79
Journal: Biosensors and Bioelectronics - Volume 81, 15 July 2016, Pages 75-79
نویسندگان
Li Zhihua, Hu Xuetao, Shi Jiyong, Zou Xiaobo, Huang Xiaowei, Zhou Xucheng, Haroon Elrasheid Tahir, Mel Holmes, Malcolm Povey,